1.斜紋夜蛾(Spodoptera litura F.)可以成功地在SL-3組半合成人工料上連續飼 養六
代以上，本飼料（SL-3組）是以Ignoffo(1963) 所使用者為主，加以修改而成， 取消
Alphacel，加入亞麻仁油(linseed o)， 並以芥藍葉粉取代棉葉粉。
在溫度27-28℃， 濕度75%-85% 條件下，由幼蟲發育至化蛹須11-14 天左右，蛹 期
7-8 天，平堩蛹重比對照組（以芥藍茶飼養）重50mg，雌蟲平均產卵數為 900-1200個
。
芥藍葉粉、抗壞血病酸，及亞麻仁油為本飼料的必要成分。抗壞血病酸的最適濃 度為
0.6%，若缺少則幼蟲在四齡前全部死亡；缺少芥藍葉粉，則幼蟲生長、發育不良 ，若
缺乏亞麻仁油則成蟲翅發育不正常而呈捲曲。
金黴素及甲基對─羥基苯為本實驗所用的防腐劑，前者之安全用量為 0.016-0.032g／
100g，而後者為0.15-0.25g／100g，若金黴素用量超過0.12g／100g，則幼蟲不 能脫
皮而滯留於一齡幼蟲期。
2.斜紋夜蛾幼蟲於接種核多角體後第四天，核多角體首先在脂肪體、真皮層、氣 管被
膜細胞、自球細胞等組織形成；第五天後，神經球、神經鞘、肌肉鞘、精巢圍鞘 ，及
中腸表皮組織等大部分的細胞核內，可以看到核多角體存在。但是在肌肉、背 管、馬
氏管及大腦等組織或器官卻找不到核多角體。感染後第六、七天，大部分的幼 蟲死亡
。
3.以n-Butanol-water phase system純化法，所得的核多角體，在電子顯微鏡鏡 下觀
察，為三角形、四角形或不規則形，其大小在1-3.5μ 之間。以鹼溶解核多角體 時，
可看到核多角體有一層薄膜，包圍著一群病毒束，每個病毒束含2-4 個病毒粒 子。病
毒粒子呈桿狀，大小約320mμ×50mμ。
4.斜紋夜蛾幼蟲的血液，其pH值為6.2； 滲透壓為359mOsm／kg， 且含有12種游 離氨
基酸，即離氨酸、組織氨酸、阿金氨酸、普氨酸、初油氨酸、纈草氨酸、甲硫氨 酸、
異白氨酸、白氨酸、酥氨酸、苯初油氨酸、甘氨酸。
SLM-1 培養液是參照TNM-FH培養液，分別加入100mg／100ml的普氨酸、氨酸及酥 氨酸
，再加10% 的斜紋夜蛾血清而成，可用來培養精巢組織達三個月之久。
接種核多角體病毒四天後，生長在SLM-1 培養液中的血球細胞及卵巢細胞，其細 胞核
內可清楚地看到核多角體形成。但精 婐的結締組織或生殖細胞並不被該核多角 體病
毒所侵染。
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1. A semi-synthetic diet (SL-3) was developed for rearing the tobacco
cutworm (Spodoptera litura F.) for over 6 continuous generations withous
deterioration. The diet was modified form Ignoffo (1963) by c ancelling
alphacel and adding inseed oil and dry-leaf powder of Chinese kale
(Brassica alboglabra) instead of cotton leaves. Under 27-28℃ and 75-85%
RH conditions, the developmental period from larval to pupal stage is
11-14 days. The period of pupa is 7-8 days. A female moth may lay
900-1,200 eggs throughout its life. Some nutritional requirements of this
moth were also studied, with dry-leaf powder of Chinese kale, ascorbic
acid and linseed oil found to be essential nutrients. The larvae might
die before the fourth instar for complete lack of ascorbic acid. Diet
without leaf-powder or linseed oil component resulted in poor development
of larvae or abnormal adult emergence.
Two antimicrobial agents were also added to the diet. The safe dosages
were 0.016-0.032g
methy1-P-hydroxybenzoate. If the dosage was increased to 0.12g
aureomycin, the larvae would not molt and would stay in its first instar.
2. Tobacco cutworm larvae infected with nuclear polyhedrosis virus were
obtained by feeding them on artificial diet (Sl-3) contaminated with
polyhedral suspension. Four days after the inoculation, polyhedra were
observed in the cells of fat body, hypodermis, treacheal matrix and
haemocytes. Five days after inoculation, polyhedra were also found in
most cells of ganglion, nerve sheath, muscle sheath, testicular peritoneal
sheath and epithelium of midgut. However, polyhedra were not observed in
cells of muscle, dorsal vessel, malpighan tubules and brain. Six or seven
days after inoculation, most of the larvae were killed by the polyhedrosis
virus.
3. The purified polyhedra were obtained by the n-Butanol - water phase
system method. Its appearance may be triangular, square or irregular in
shape and about 1.0-3.5μ diameter. The polyhedra were found to have a
membrane enclosing a number of virus bundles. Each bundle consists of 2-4
virus particles. The size of virus particle is approximately 320mμ×
520mμ.
4. The PH value and osmotic pressure of tthe tobacco cutworm blood is
approximately 6.2 and 359 m OSM
i.e., lysine, histidine, arginine, proline, glycine, alanine, valine,
methionine, isoleucine, leucine, tyrosine and phenylanine, were identified
in the blood. The SLM-1 medium was proline and lysine tyrosine and by
adding 10% homologus haemolymph. The medium was used for primary culture
of the haemocytes and ovarian cells, which were maintained for about one
month by changing the medium once every four days. SLM-2 medium was a
mixed medium, i.e., onhe part of G.M.A. plus one part of M.M. medium added
with 10% homologous haemolymph. The testicular cells had been cultured in
this medium for three months. Four days after inoculation of virus, the
polyhedra could be found in the nuclei of haemocytes and ovarian cells
growing on SLM-1 medium. However, the virus did not infect the male germ
cells and connective tissue resulting from testicular cyst walls.

100g in aureomycin and 0.15-0.25g
100g of

kg, respectively. Twelve amino acids,