1.蜜蜂微粒子病，為成蜂之重要病害。其病徵為腹部膨脹，不善飛翔，前後翅之 翅釣
不連接，在蜂箱前的草地上爬行；取出病蜂的腸首，中腸之環形節狀消失，呈灰 白色
，較正常蜜蜂之中腸膨大。蜂箱門口採集之病蜂，鏡檢其腸道磨碎懸浮液87.5％ 含有
微粒子病孢子。故檢查蜂群是否罹病，簡便法為取出腸道觀察其是否膨大變色。 但正
可靠的檢查，必須配合顯微鏡，檢查病原孢子。
2.微粒子病孢子在光學顯微鏡下，呈橢圓形顆粒狀，屈光性很高的粒子。大小為
4.7-6.4μ×2.6-3.4μ平均大小為：5.7μ×3.0μ。在掃描式電子顯微鏡下，孢 子外
表光滑，無任何突起。
3.自病蜂體內分離出之微粒子病孢子，以（圖表省略）
五種劑量餵飼接種第四日齡蜜蜂；接種後第十三天之平均累積死亡率，依次為 54.17
；64.17；77.50；83.33；94.17。病蜂之壽命較健康蜜蜂縮短21.3-41.9 ％。
4.經組織怍片觀察，接種病原後第二天在密蜂中腸上皮細胞中可見雙核游走 子 ，第三
、四天形成為裂殖體，第五、六天形成四核體。由此推知病原孢子在寄主體中完 成一
世代，約需5-6 天。
5.微粒子病在本省的蜂群中，形成春末夏初及秋季二發生盛期。南部（高雄）比 北部
（台北）的溫度較高，降雨較少，故罹病率較低，氣溫及降雨量的變化影響發病 率，
為影響病害的重要因子。
6.微粒子病罹病蜂群，蜂王乳顯著減收，平均每一王台減少0.013 公克，易被養 蜂者
忽略。
7.蜂王寶對微粒子病之防治，分別於室內蜂場之試驗，證明皆有良好之效果。本 省之
單體式蜂群中，於秋季及冬末春初，每群蜜蜂連續餵飼十次600ml 的含藥糖水，
Fumagillin含量為25mg
燈消毒，更新巢脾，以冰醋酸之消毒等處理，使防治的效果增加。
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1.Nosema disease is a ser ious world-wide disease of honey bees. It is
caused by Nosema apis Zander, a microsporidian parasite of the intestinal
epithelium of adult bees. Infected adults appear to be sluggish. The
wings are disjointed and incapable of normal flight. The midgut of the
alimentary tract of an infected bee is white and opaque in appearance, and
somewhat swollen, while the midgut of the healthy bee is amber and
translucent. This is a simple and convenient method to identify the
Nosema infection in the apiary. Furthermore, the definite diagnosis of
the disease is confirmed by examining the spore of the suspension of
abdominal contents by microscope.
2.The spore of Nosema is usually oval in shape and highly refractile. The
range of lengty and width are 4.7-6.4u X 2.6-3.4 with 5.7X3.0u in average.
The spore membrane is not a rigid structure under the scanning electron
microscopic examination.
3.A laboratory study was carried out as following:four-day-old worker
honey bees were divided as 6 groups. One group of bees were fed 33%
sucrose solution only. The other 5groups, bees were fed individually with
5 ul 33% sucrose solution with （圖表省略）
Nosema spore, respectively. The bees were then transferred to the cages
in the laboratory. Fifteer days after treatment, the accumulative
mortality were 30.00%,54.17%,64.17%,83.33%,94.17% in each treated group.
The longevity of adult bees was shortened about 21.3-41.9% after the
treatments.
4.The sections of the midgut of the infected bees examined histologically
by light microscope after the innoculation. Binucleate plenonts had
become embeded in the host epithelial cells within two days, schizonts
were found in three to four days. The four-nucleate stage appeared
abundently at five to six days. The spore completed life cycle in the
host epithelial cells for a period of approzimately five to six days .
5.A survey of incidence of Nosema disease of the honey bees has shown that
two periods of infection occured in Taiwan. One period is in the late
spring and earily summer, the other period is in sutumn. The average
temperature in south (Kaohsing) is higher than in north (Taiper) of this
island. The level of Nosema infection at south is lower than at norther
region. Temperature and precipitation might be factors which influence
the level of Nosema infection.
6.The average production of royal jelly of a single artificial queen cell
in Nosema infected colonies decreased 0.013 g compared to the health
colonies. It is too difficult to detect by experienced beekeepers,
sometimes this small amountof loss is easily overlooked.
7.Each Tai-type colony, a single hive body with 9 frames, was fed with
600ml of sucrose sloution contain 25 mg
from both tests in the laboratory and theapiary show that the level of
Nosema infection was reduces by the treatment.

l，為正確的使用劑量。蜂場使用時，厄合蜂箱用高溫汽 油噴

l of fumagillin. the results